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Cholesterol Esterase (CHE-XE) Cholesterol Esterase (CHE-XE) The enzyme is useful for the determination of cholesterol in clinical analysis. S000205 Hóa chất phân tích Số lượng: 0 cái

Cholesterol Esterase (CHE-XE)

  • The enzyme is useful for the determination of cholesterol in clinical analysis.
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Origin recombinant E. coli
Lineage name

Steryl-ester acylhydrolase

EC Number 3.1.1.13
Reaction formula

Cholesterol ester + H2O →→→→ Cholesterol + Fatty acid

SPECIFICATION

Appearance light yellow lyophilizate  
Activity ≧5 U/mg lyophilizate  
Stabilizer sucrose  
Storage below -20℃  

PROPERTIES

Molecular weight ca. 54 kDa (gel filtration)
Structure monomer of 54 kDa (SDS-PAGE)
Michaelis constant 1.9×10-5 M (cholesterol linoleate)
pH Optimum 5.5–7.0
pH Stability 5.0–10.0
Optimum temperature 40℃
Thermal stability below 55℃
Specificity cholesterol linoleate (100), cholesterol acetate (2)
cholesterol oleate (98), cholesterol palmitate (74)
cholesterol stearate (68), cholesterol arachidonate (46)

APPLICATIONS

The enzyme is useful for the determination of cholesterol in clinical analysis.

REFERENCES

  • Richmond, W., Clin. Chem., 19, 1350 (1973)
  • Flegg, H. M., Ann. Clin. Biochem., 10, 79 (1973).
  • Alain, C. C. et al., Clin. Chem.,20, 470 (1973).
  • Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724 (1974).
  • Kameno. Y. et al., Jap. J. Clin. Path., 24, 650 (1976).

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