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Creatininase (C1- E) Creatininase (C1- E) The enzyme is useful for the determination of creatinine in clinical analysis. S000200 Hóa chất phân tích Số lượng: 0 cái

Creatininase (C1- E)

  • The enzyme is useful for the determination of creatinine in clinical analysis.
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Origin recombinant E. coli
Lineage name

Creatinine amidohydrolase

EC Number 3.5.2.10
Reaction formula

Creatinine H2O Creatine

SPECIFICATION

Appearance yellow lyophilizate  
Activity ≧500 U/mg lyophilizate  
Contaminant catalase ≦1.0%  
Stabilizer sucrose  
Storage below -20℃  

PROPERTIES

Molecular weight ca. 170 kDa (gel filtration)
Structure  6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point 4.8
Michaelis constant 3.4×10-2M (creatinine)
  4.3×10-2M ((creatine)
pH Optimum 6.5–7.0
pH Stability 7.0–11.0
Optimum temperature  60–65℃
Thermal stability below 60℃
Stability (liquid form) stable at 37°C for at least two weeks 
Stability (powder form) stable at 30°C for at least one month
Inhibitors Hg2+
Activators Mg2+,Mn2+ 

APPLICATIONS

The enzyme is useful for the determination of creatinine in clinical analysis.

REFERENCES

Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984)


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