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Fructosyl-amino Acid Oxidase (FAOX-TE) Fructosyl-amino Acid Oxidase (FAOX-TE) The enzyme is useful for the determination of fructosyl-L-amino acid. S000199 Hóa chất phân tích Số lượng: 0 cái

Fructosyl-amino Acid Oxidase (FAOX-TE)

  • The enzyme is useful for the determination of fructosyl-L-amino acid.
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Origin recombinant E. coli
Lineage name

Fructosyl-L-amino acid : oxygen oxidoreductase

EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2 →→→ L-Amino acid + Glucosone + H2O2

SPECIFICATION

Appearance yellow lyophilizate  
Activity ≧1.0 U/mg lyophilizate  
Stabilizer trehalose  
Storage below -20℃ protected from light  

PROPERTIES

Molecular weight ca. 88 kDa (gel filtration)
Structure 2 subunits of 44 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 5.0×10-4M (fructosyl-glycine)
pH Optimum 7.5–8.5
pH Stability 6.0–10.0
Optimum temperature 40–50℃
Thermal stability below 45℃
Stability (liquid form) stable at 20℃ for at least two weeks
Stability (powder form) stable at 37℃ for at least one month
Inhibitors Hg2+,Pb2+
Specificity fructosyl-glycine (100), fructosyl-L-valine (132)
Nε-fructosyl-L-lysine (0)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-L-amino acid.

REFERENCES

  • Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
  • Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).
  • Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).
  • Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).
  • Sakaue, R. et al., Acta Cryst., F61, 196–198 (2005).

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