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Ms. Như

Fructosyl-peptide Oxidase (FPOX-CE) Fructosyl-peptide Oxidase (FPOX-CE) The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid. S000197 Hóa chất phân tích Số lượng: 0 cái

Fructosyl-peptide Oxidase (FPOX-CE)

  • The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.
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Origin recombinant E. coli
Lineage name

Fructosyl-peptide : oxygen oxidoreductase

EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

SPECIFICATION

Appearance yellow lyophilizate  
Activity

≧6.04 U/mg lyophilizate

 
Stabilizer EDTA, glutamate  
Storage below -20℃ protected from light  

PROPERTIES

Molecular weight ca. 60kDa (gel filtration)
Structure monomer of 52 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 3.4×10-4M (fructosyl-valyl-histidine)
4.4×10-4M (fructosyl-glycine)
8.9×10-4M (Nε-fructosyl-lysine)
pH Optimum 7.5–8.0
pH Stability 6.0–9.5
Optimum temperature 35–42℃
Thermal stability below 45℃
Stability (powder form) stable at 37℃ for at least one month
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine (53)
Nε-fructosyl-lysine (84)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

REFERENCES

  • Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
  • Hirokawa, K. et al., Arch. Microbiol., 180, 227–231 (2003).
  • Hirokawa, K. et al., Biochem. Biophys. Res. Commun., 311, 104–111 (2003).

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