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Mr. Nam

Ms. Như

Glucose Dehydrogenase (FADGDH-AA) Glucose Dehydrogenase (FADGDH-AA) The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters. S000195 Hóa chất phân tích Số lượng: 0 cái

Glucose Dehydrogenase (FADGDH-AA)

  • The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
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Origin recombinant A. sojae
Lineage name

D-Glucose : acceptor 1-oxidoreductase

EC Number 1.1.5.9
Reaction formula

D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor

SPECIFICATION

Appearance Yellow lyophilizate  
Activity ≧500 U/mg lyophilizate  
Contaminant NAD Glucose Dehydrogenase  ≺1.0×10-2%  
Hexokinase ≺1.0×10-2%  
α-Glucosidase ≺1.0×10-2%  
β-Glucosidase ≺1.0×10-2%  
Storage below -20℃ protected from light  

PROPERTIES

Molecular weight ca. 90 kDa (gel filtration)
Structure monomer, one mole of FAD per mole of enzyme glycoprotein
Michaelis constant 9.5×10-2M (d-Glucose)
pH Optimum 7.0–7.5
pH Stability 2.5–7.5
Optimum temperature 40–50℃
Thermal stability below 50℃
Inhibitors Ag2+
Specificity d-glucose (100), Maltose (≺1), 
d-xylose (≺1), d-galactose (≺1) 

APPLICATIONS

The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.

REFERENCES

Satake, R. et al., J Biosci Bioeng., 120, 498–503 (2015)


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