Mr. Quang

Mr. Nam

Ms. Như

Lactate Dehydrogenase (LDH-P) Lactate Dehydrogenase (LDH-P) The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes. S000191 Hóa chất phân tích Số lượng: 0 cái

Lactate Dehydrogenase (LDH-P)

  • The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
Lĩnh vực
Hãng cung cấp
 
Origin porcine heart
Lineage name

(S )-Lactate : NAD+ oxidoreductase

EC Number 1.1.1.27
Reaction formula

Pyruvate + NADH + H+ L-Lactate + NAD+

SPECIFICATION

Appearance white lyophilizate  
Activity ≧150 U/mg lyophilizate  
Contaminants GPT ≦1.0×10-2%  
malate dehydrogenase ≦5.0×10-2%  
pyruvate kinase ≦1.0×10-2%  
Storage below -20℃  

PROPERTIES

Molecular weight ca. 115 kDa (gel filtration)
Michaelis constant 2.5×10-2M (fructosyl-glycine) 
1.0×10-4M (pyruvate)
pH Optimum 7.4–8.0
pH Stability 7.0–7.5
Optimum temperature 50–55℃
Thermal stability below 45℃

APPLICATIONS

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

REFERENCES

  • Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
  • Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
  • Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
  • Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).

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