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Sucrose Phosphorylase (SPL-E) Sucrose Phosphorylase (SPL-E)

The enzyme is useful for the determination of inorganic phosphate in clinical analysis.

 
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Sucrose Phosphorylase (SPL-E)

  • The enzyme is useful for the determination of inorganic phosphate in clinical analysis.

     
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Origin recombinant E. coli
Lineage name

Sucrose : orthophosphate α-D-glucosyltransferase

EC Number 2.4.1.7
Reaction formula

Sucrose + Orthophosphate →→→ D-Fructose + α-D-Glucose 1-phosphate

SPECIFICATION

Appearance white lyophilizate  
Activity ≧50 U/mg lyophilizate  
Contaminants NADH oxidase ≦5.0×10-4%  
6-phosphogluconate dehydrogenase ≦5.0×10-4%  
Stabilizer sucrose  
Storage at-20℃  

PROPERTIES

Molecular weight ca. 56 kDa (gel filtration)
Structure monomer of 56 kDa (SDS-PAGE)
Isoelectric point 4.6
Michaelis constant 3.9×10-2M (sucrose)
6.2×10-3M (phosphate)
pH Optimum 7.5
pH Stability 5.0–8.0
Optimum temperature 40℃
Thermal stability below 45℃
Stability (liquid form) stable at 37℃ for at least two weeks
Stability (powder form) stable at 30℃ for at least two weeks
Inhibitors glucose, glucono-1,5-lactone
Specificity sucrose (100), maltose (0), starch (0)

APPLICATIONS

The enzyme is useful for the determination of inorganic phosphate in clinical analysis.

REFERENCES

  • Koga, T. et al., Agric. Biol. Chem., 55, 1805–1810 (1991).
  • Kitao, S. and Nakano, E., J. Ferment. Bioeng., 73, 179–184 (1992).

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