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Uricase (U-TE) Uricase (U-TE) The enzyme is useful for the determination of uric acid in clinical analysis. S000185 Hóa chất phân tích Số lượng: 0 cái

Uricase (U-TE)

  • The enzyme is useful for the determination of uric acid in clinical analysis.
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Origin recombinant E. coli
Lineage name

Urate : oxygen oxidoreductase

EC Number 1.7.3.3
Reaction formula

Urate + H2O + O2 →→→ Unidentified products + H2O2

             ↓

             CO2 + Allantoin (racemic mixture)

SPECIFICATION

Appearance light brownish lyophilizate  
Activity ≧4U/mg lyophilizate  
Contaminant catalase ≦1.0%  
Stabilizer sucrose,citrate,EDTA  
Storage below -20℃  

PROPERTIES

Molecular weight ca. 90 kDa (gel filtration)
Structure 2 subunits of 35 kDa (SDS-PAGE)
Michaelis constant 1.1×10-5M (uric acid)
pH Optimum 8.5
pH Stability 7.0–11.0
Optimum temperature 45℃
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least ten days
Stability (powder form) stable at 30℃ at least three weeks
Inhibitors Hg2+,Ag+

APPLICATIONS

The enzyme is useful for the determination of uric acid in clinical analysis.

REFERENCES

Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).


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