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RIDASCREEN® Nitrofuran (AHD) RIDASCREEN® Nitrofuran (AHD)

RIDASCREEN® Nitrofuran (AHD) is a competitive enzyme immunoassay for the quantitative analysis of the nitrofurantoin metabolite AHD in shrimp and fish.

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RIDASCREEN® Nitrofuran (AHD)

  • RIDASCREEN® Nitrofuran (AHD) is a competitive enzyme immunoassay for the quantitative analysis of the nitrofurantoin metabolite AHD in shrimp and fish.

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General information:

Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently used in animal production due to their excellent antibacterial and pharmacokinetic properties. They have also been  used as growth promoters during the production of shrimp, poultry and pigs. Long term animal experiments have shown that the parent compounds and their metabolites have carcinogenic and  mutagenic characteristics. This led to the prohibition of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin and nitrofurazone for use in animals used as sources of food, and in 1995 the use of furazolidone was also prohibited.

Specifications

Art. No. R3713
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation homogenization, derivatization, extraction, centrifugation, evaporation and defatting

A detailed Application Note, describing an uniform sample preparation for shrimp and fish with the ability to analyze the same sample extract in all 4 RIDASCREEN® Nitrofuran ELISA tests, is available on request.
Incubation time 1 h 15 min
Detection limit Shrimp: approx. 200 ng/kg (ppt)
Fish: approx. 76 ng/kg (ppt)

(corresponding to the standard substance)
Cross reactivity

Nitrophenyl- (NP) AHD: 100 %
NP-AMOZ, NP-AOZ, NP-SEM:  < 1 %
Nitrofurantoine :  approx. 32 %
Furaltadone, Furazolidone, Nitrofurazone: < 2 %
AHD, AMOZ, AOZ, SEM:  < 1 %

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