| Origin | recombinant A. sojae |
|---|---|
| Lineage name | D-Glucose : acceptor 1-oxidoreductase |
| EC Number | 1.1.5.9 |
| Reaction formula | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Glucose Dehydrogenase (FADGDH-AA)
- The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
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SPECIFICATION
| Appearance | Yellow lyophilizate | |
|---|---|---|
| Activity | ≧500 U/mg lyophilizate | |
| Contaminant | NAD Glucose Dehydrogenase ≺1.0×10-2％ | |
| Hexokinase ≺1.0×10-2％ | ||
| α-Glucosidase ≺1.0×10-2％ | ||
| β-Glucosidase ≺1.0×10-2％ | ||
| Storage | below -20℃ protected from light |
PROPERTIES
| Molecular weight | ca. 90 kDa (gel filtration) |
|---|---|
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 9.5×10-2M (d-Glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 2.5–7.5 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 50℃ |
| Inhibitors | Ag2+ |
| Specificity | d-glucose (100), Maltose (≺1), |
| d-xylose (≺1), d-galactose (≺1) |
APPLICATIONS
The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
REFERENCES
Satake, R. et al., J Biosci Bioeng., 120, 498–503 (2015)
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