| Origin | recombinant E. coli |
|---|---|
| Lineage name | Creatine amidinohydrolase |
| EC Number | 3.5.3.3 |
| Reaction formula | Creatine + H2O →→→ Sarcosine + Urea |
Creatinase (C2-AT)
- The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
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SPECIFICATION
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧9 U/mg lyophilizate | |
| Contaminant | catalase ＜0.5% | |
| Stabilizer | sucrose | |
| Storage | below -20℃ |
PROPERTIES
| Molecular weight | ca. 80 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 48 kDa (SDS-PAGE) |
| Michaelis constant | 8.6×10-3M (creatine) |
| pH Optimum | 7.0–9.0 |
| pH Stability | 4.0–11.0 |
| Optimum temperature | 45℃ |
| Thermal stability | below 53℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Hg2+ |
APPLICATIONS
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
REFERENCES
- Suzuki, M., Medical Technology, 7, 945–950 (1979).
- Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 140, 289–294 (1984).
- Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).
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