Sample Preparation
For indicator organisms (TC, ETB, YM and XSA):
For indicator organisms (TC, ETB, YM and XSA):
- Water Soluble Samples – Weigh 10g of sample and dilute with 90mL Modified Letheen Broth (MLB).
- Oil-based Samples and Powders – Weigh 10g of sample and dilute with 80 mL sterile MLB supplemented with 10 mL Tween (Polysorbate) 80 or 20, then homogenize.
Regulatory Limits
Refer to the United Pharmacopeia (USP) or other local regulatory bodies.
Refer to the United Pharmacopeia (USP) or other local regulatory bodies.
Processing Notes
The addition of Tween (Polysorbate) not only aids in dispersion of non-water soluble samples, but also acts as a neutralizer to the antimicrobial agents found in cosmetics.
Effect of Neutralizer to Result Interpretation
MLB is necessary to neutralize antimicrobial compounds in the formulation. Using other diluent without neutralizer, e.g Butterfield’s Phosphate Buffer (BPB) in Figure 2, will result to poor recovery of the target organism.
Effect of Sample Color to result interpretation
Color of the sample may interfere with the interpretation of the results (Figure 3). Plate the highest dilution possible without affecting the needed sensitivity of the test, e.g. if allowable level for total count is 500 CFU/g, plate 1mL of the 10-2 dilution (Figure 4) but not 10-3 since it cannot detect the required level.
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
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