Sample Preparation
- For indicator organisms (TC, EC, ETB, YM, XSA and PA) – Weigh 10g and dilute with buffered diluent supplemented with tween (polysorbate) 80 or 20 (e.g. Butterfield’s Phosphate Buffer (BPB), Fluid Soybean Casein Digest Medium, or Tryptic Soy Broth (TSB). Dilute as needed.
- For Pathogens (SL and LS) – Weigh 25g and dilute with 225ml sterile TSB
Regulatory Limits
Refer to the United States Pharmacopeia (USP), Food & Drug Administration (FDA), or other local regulatory bodies.
Refer to the United States Pharmacopeia (USP), Food & Drug Administration (FDA), or other local regulatory bodies.
Processing Notes
The addition of Tween or Polysorbate (4% v/v) not only aids in dispersion of non-water soluble samples, but also acts as a neutralizer to the antimicrobial agents found naturally in most dietary supplements of plant origin.
Effect of sample color to result interpretation
Color of the sample may interfere with the interpretation of the results. Plate the highest dilution possible without affecting the needed sensitivity of the test. For sample, if allowable level for total count is 200 CFU/g, plate 1mL of the 10-2 dilution but not 10-3 since it cannot detect the required level.
Similar to other tests that rely on β-Glucuronidase activity to detect E.Coli, CompactDryTM EC cannot detect E.Coli O157:H7 as an E.Coli.
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
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