Compact Dry Application in Fresh Produce Industry | Nissui
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Commonly tested parameters:
Total Count (TC), Ecoli and Coliform (EC), Enterobacteriaceae (ETB), Yeast and Mold - (YMR), Staphylococcus aureus (XSA), Salmonella (SL) and Listeria (LS).
Sample Preparation
For indicator organisms (TC, EC, ETB, YMR, and XSA) - cut out standrardized sample size (e.g 10 g or 25g) from the fruit or vegetable using sterile knife or scissors, then place in sterile container. Add the appropriate volume of sterile diluent (e.g 90ml or 225ml of Buffered peptone water or BPW, Maximum Recovery Diluent or MRD, or 0.1% Peptone water), then Homogenize and dilute accrordingly
For Pathogens (SL and LS) – For cut samples, weigh 25g and dilute with 225ml sterile BPW. For whole fruits or vegetables, test samples as is, including any visible dirt. Add sufficient sterile BPW to float the sample.
Regulatory limits
Refer to codex Alimentarius, Food & Drug Administration (FDA), Food Standards Agency (FSA), or other local regulatory bodies.
Processing Notes
Check sample pH after the 10-1 dilution especially for acidic fruits. Ensure that sample pH is between 6 and 8, otherwise neutralize using sterile 0.1N Sodium Hydroxide (NaOH) if lower than 6, or 0.1N Hydrochloric Acid (HCl) if higher than 8.
For CompactDryTM EC, interference may be observed as most fruits (e.g apples and tomatoes) naturally have Beta-Galactosidase. Dilute accordingly.
For indicator organisms (TC, EC, ETB, YMR, and XSA) - cut out standrardized sample size (e.g 10 g or 25g) from the fruit or vegetable using sterile knife or scissors, then place in sterile container. Add the appropriate volume of sterile diluent (e.g 90ml or 225ml of Buffered peptone water or BPW, Maximum Recovery Diluent or MRD, or 0.1% Peptone water), then Homogenize and dilute accrordingly
For Pathogens (SL and LS) – For cut samples, weigh 25g and dilute with 225ml sterile BPW. For whole fruits or vegetables, test samples as is, including any visible dirt. Add sufficient sterile BPW to float the sample.
Regulatory limits
Refer to codex Alimentarius, Food & Drug Administration (FDA), Food Standards Agency (FSA), or other local regulatory bodies.
Processing Notes
Check sample pH after the 10-1 dilution especially for acidic fruits. Ensure that sample pH is between 6 and 8, otherwise neutralize using sterile 0.1N Sodium Hydroxide (NaOH) if lower than 6, or 0.1N Hydrochloric Acid (HCl) if higher than 8.
For CompactDryTM EC, interference may be observed as most fruits (e.g apples and tomatoes) naturally have Beta-Galactosidase. Dilute accordingly.
Like other tests that rely on β-Glucuronidase activity to detect E.Coli, CompactDryTM EC cannot detect E.coli O157:H7 as an E.coli.
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/vi/shops/dia-compact-dry/dia-kiem-tra-vi-khuan-nhanh-compact-dry-nissui.html
All information about Compact Dry products, customers can see at:
http://azlab.vn/vi/shops/dia-compact-dry/dia-kiem-tra-vi-khuan-nhanh-compact-dry-nissui.html
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