Sample Preparation
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
- For indicator organisms (TC, EC, ETB, YM, XSA and XBC) - cut out standrardized sample (e.g 10 g or 25g) using knife of scissors. Place in sterile container, then add the appropriate volume of sterile diluent (e.g 90ml or 225ml of Buffered peptone water or BPW, maximum recovery Diluent or MRD, or 0.1% Peptone water). Homogenize and dilute accrordingly.
- For pathogens (SL)- Weigh 25g and dilute with 225ml sterile BPW.
Regulatory limits
Refer to codex Alimentarius, Food and Drug Administrator (FDA), Food Standards Agency (FSA), or other local regulatory bodies.
Processing notes
- For pathogens (SL)- Weigh 25g and dilute with 225ml sterile BPW.
Regulatory limits
Refer to codex Alimentarius, Food and Drug Administrator (FDA), Food Standards Agency (FSA), or other local regulatory bodies.
Processing notes
- Use 25g sample size for samples that absorb a large volume of diluent (e.g water and biscuits).
- User filter bags to minimize inclusion of sample particles during inoculation.
- Check sample pH after the 10-1 dilution especially for acidic fruits. Ensure that sample pH is between 6 and 8, otherwise neutralize using sterile 0.1N Sodium Hydroxide (NaOH) if lower than 6, or 0.1N Hydrochloric Acid (HCl) if higher than 8.
Effect of sample color to result interpretation
Color of the sample may interfere with the interpretation of the results. Plate the highest dilution possible without affecting the needed sensitivity of the test. For sample, if allowable level for aerobic count is 800 CFU/g, plate 1mL of the 10-2 dilution but not 10-3 since it cannot detect the required level.
Color of the sample may interfere with the interpretation of the results. Plate the highest dilution possible without affecting the needed sensitivity of the test. For sample, if allowable level for aerobic count is 800 CFU/g, plate 1mL of the 10-2 dilution but not 10-3 since it cannot detect the required level.
Similar to other tests that rely on β-Glucuronidase activity to detect E.Coli, CompactDryTM EC cannot detect E.coli O157:H7 as an E.coli.
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
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